ZFIN Image: Testa et al., 2024, Figure 4

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Figure 4

Analysis of the angiogenic potential of microglial EVs in zebrafish. (A) Schematic of the experimental set-up. EVs were grafted with Matrigel in the proximity of the developing sub-intestinal vein (SIV), as shown by a black asterisk, and vascular sprouting from the SIV was assessed after 24 h. The area of vessel analysis is represented by a blue square. (B) Histograms represent the number of vessels sprouting towards the implant, counted for each embryo, divided into categories (no vessels, 1–2 vessels, >3 vessels). Data are expressed as the percentage of embryos in each category (n = 36 embryos/group). (C) Representative confocal images showing whole-mount alkaline phosphatase (AP) staining of embryos at 1 dpi in lateral view. Injection of hypEVs stimulated the migration of SIV-derived vascular sprouts towards the implant, while injection with contrEVs resulted in a normal development of the vascular plexus. Chi-Square test was performed. ** p < 0.01.

Acknowledgments

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