ZFIN Image: Nilles et al., 2024, Fig. 3

Image

Figure Caption

Fig. 3 Neomycin dose response in YO-PRO-1-stained AB fish. (A) Top: Representative neomycin dose-response curve generated by PEPITA with relative fluorescence units (RFU) (EC50 = 1.4 μM). Middle and bottom: Representative image of a fish exposed to no drug (middle) and exposed to 2.5 μM neomycin (bottom), both used for PEPITA quantification. (B) Top: representative neomycin dose-response curve generated by standard approach of counting HCs from individual neuromasts (EC50 = 2.0 μM). Middle and bottom: Representative image of an individual neuromast from a fish exposed to no drug (middle) and exposed to 2.5 μM neomycin (bottom), used for HC counting. Note that PEPITA uses whole-fish images for quantification; images have been cropped for better visualization of the stained neuromasts. Scale bars: fish images = 300 μm; neuromast images = 10 μm.

Acknowledgments

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