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Fig. 2 Characterization of the EVs. A Western blot for tetraspanins (CD63, CD81, CD9), TSG101 and RPS6 was performed on EVs purified by immunoprecipitation and derived from MDA-MB-453, MCF-7, MDA-MB-231, and HCC1937 cells treated with sorafenib. B Transmission electron microscopy (TEM) on EVs obtained by ultracentrifugation showed vesicles with characteristic morphology and size, between 50 and 150 nm in diameter. Scale bar, 100 nm
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