Fig. 6.

Fig. 6.

Expression of representative myopia-associated genes for efemp1^+/+ and efemp1^2C-Cas9 fish after 2 days or 4 weeks of L/D or D/D rearing. (A) efemp1^+/+ and efemp1^2C-Cas9 fish were reared under a standard L/D to 4 wpf and then were reared under L/D or D/D to up to 8 wpf (4 weeks of treatment). Eyes for molecular analysis were sampled after 2 days or 4 weeks of treatment. Relative mRNA levels of myopia-associated genes, including (B) efemp1, (C) egr1, (D) tgfb1a, (E) tgfb1b, (F) vegfaa, (G) vegfab, (H) igf1, (I) fgf2, (J) wnt2b, (K) rbp3, (L) gjd2a, (M) and gjd2b were analyzed for efemp1^+/+ and efemp1^2C-Cas9 fish after 2 days and 4 weeks of treatment (n=4 samples per group), using RT-qPCR. Group data are shown as mean±s.e.m. Three-way ANOVA and Fisher's LSD post-hoc tests were performed. Significant main effects of genotypes (G), rearing conditions (E) and their interactions (G×E) are indicated in the graphs. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.