Fig. 4.

Fig. 4.

Ocular refraction of efemp1^+/+ and efemp1^2C-Cas9 fish after environmental treatment. (A) Efemp1^+/+ and efemp1^2C-Cas9 fish were reared under normal light/dark cycle (L/D) until 4 weeks post-fertilization (wpf) and either continued under normal lighting (L/D) or switched to dark-rearing (D/D) for 2 or 4 weeks (to 6 or 8 wpf, respectively). (B) Relative ocular refraction of L/D- or D/D-reared efemp1^+/+ and efemp1^2C-Cas9 eyes were quantified after 2 or 4 weeks of treatment using optical coherence tomography (OCT). There were 39, 40, 42 and 40 eyes at the 2-week time point, and 40, 34, 40 and 40 eyes at the 4-week time point for L/D-reared efemp1^+/+, L/D-reared efemp1^2C-Cas9, D/D-reared efemp1^+/+, D/D-reared efemp1^2C-Cas9 fish, respectively. Group data are shown as mean±s.e.m. Three-way ANOVA and Fisher's LSD post-hoc analyses were performed. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.