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Fig. 7 NMP cells’ molecular fingerprints extracted from the scRNA-seq, related to Figure 6 (A) Selection of NMPs enriched genes for signaling pathway important for vertebrates’ axial elongation, showing a peak of expression early during development. (B) Zebrafish oct4 (pou5f3) timeline of spatial expression in the posterior body clusters shows late maintenance of oct4, specifically in the NMP cells. (C) Selection of essential genes for cell cycle enriched in the early NMPs. (D) UMAP embedding of the NMPs cluster in the early integrated UMAP (10, 12, 14, 16, 19, and 24 hpf) color coded by time point (top left). The same embedding is then color coded for the NMP molecular signature genes (sox2 and tbxta) and three important genes for notochord development. (E) A dot plot of the zebrafish hox genes’ expression over time in the NMP clusters shows a collinear activation of the hox pathway.
Reprinted from Cell, 187(23), Lange, M., Granados, A., VijayKumar, S., Bragantini, J., Ancheta, S., Kim, Y.J., Santhosh, S., Borja, M., Kobayashi, H., McGeever, E., Solak, A.C., Yang, B., Zhao, X., Liu, Y., Detweiler, A.M., Paul, S., Theodoro, I., Mekonen, H., Charlton, C., Lao, T., Banks, R., Xiao, S., Jacobo, A., Balla, K., Awayan, K., D'Souza, S., Haase, R., Dizeux, A., Pourquie, O., Gómez-Sjöberg, R., Huber, G., Serra, M., Neff, N., Pisco, A.O., Royer, L.A., A multimodal zebrafish developmental atlas reveals the state-transition dynamics of late-vertebrate pluripotent axial progenitors, 6742-6759.e17, Copyright (2024) with permission from Elsevier. Full text @ Cell