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Fig. S6 Tissue morphodynamics constrain the state transition of NMPs during vertebrate body axis elongation, related to Figure 4 (A) Sketch of the dynamic morphoskeleton approach used to identify robust dynamical features from multicellular flows. We focus on repellers—a type of Lagrangian coherent structure locating divergent cell trajectories. We identify repellers by computing the Lagrangian stretching rate ( see Supplemental theory note). (B) Illustration of the time intervals selected to compute the stretching rates, and anatomical annotations of the tail’s territories. NMP, neural and mesodermal tissues. (C) Vector fields representing the cells’ motion during these time intervals, and dashed lines representing the presumptive NMP territory. (D) Lagrangian stretching rate (brighter shades correspond to more stretching, i.e., divergent cell trajectories) and the emergence of repellers in the early NMP presumptive territory. This territory was ascertained by HCR and is outlined by a dashed line.
Reprinted from Cell, 187(23), Lange, M., Granados, A., VijayKumar, S., Bragantini, J., Ancheta, S., Kim, Y.J., Santhosh, S., Borja, M., Kobayashi, H., McGeever, E., Solak, A.C., Yang, B., Zhao, X., Liu, Y., Detweiler, A.M., Paul, S., Theodoro, I., Mekonen, H., Charlton, C., Lao, T., Banks, R., Xiao, S., Jacobo, A., Balla, K., Awayan, K., D'Souza, S., Haase, R., Dizeux, A., Pourquie, O., Gómez-Sjöberg, R., Huber, G., Serra, M., Neff, N., Pisco, A.O., Royer, L.A., A multimodal zebrafish developmental atlas reveals the state-transition dynamics of late-vertebrate pluripotent axial progenitors, 6742-6759.e17, Copyright (2024) with permission from Elsevier. Full text @ Cell