Fig. 4

Fig. 4

Cisplatin treatment decreased neuromast hair cell numbers more than compound B in brn3c(pouf4f3):eGFP zebrafish A.-F. Representative images of control and experimental compound (A., 0.3% DMSO; B., 0.3 µM compound B; C., 0.9% NaCl; D., 2 µM cisplatin; E., E3 media; F., 100 µM cisplatin) treated brn3c (pouf4f3):eGFP zebrafish embryos. G. Graph of average number of neuromast hair cells counted for control and experimental compound treated zebrafish embryos. Arrow in A. indicates neuromast expressing GFP reporter. Key: Con = E3 egg water media control; C = cisplatin; C-V = cisplatin 0.9% NaCl vehicle control; B = compound B; B-V = 0.3% DMSO compound B vehicle control; ↑C = 100 µM cisplatin overdose used as a positive control; p < 0.05, “*” = 0.05, “**” = 0.01, “****” = 0.0001, ns = non-significant