Fig. 3

Fig. 3

Cisplatin and compound B treatment caused increased general tissue cell apoptosis in zebrafish AB embryos A.-F. Representative images of control and experimental compound (A., 0.3% DMSO; B., 0.3 µM compound B; C., 0.9% NaCl; D., 2 µM cisplatin; E., E3 media; F., 10 nM CCCP) treated zebrafish embryos). G. Graph of average number of apoptotic cells observed for control and experimental compound treated zebrafish embryos. Arrow in A. indicates caspase-3 positive cell. Key: Con = E3 egg water media control; C = cisplatin; C-V = cisplatin 0.9% NaCl vehicle control; B = compound B; B-V = 0.3% DMSO compound B vehicle; CCCP = 10 nM carbonyl cyanide m-chlorophenyl hydrazine positive control; p < 0.05, “*” = 0.05, “****” = 0.0001