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Fig. 9 Generation of ctnnb2 maternal mutants using this protocol. These data were reproduced from our previous publication [12]. A. Phenotypes of the GFP-positive embryos expressing three sgRNAs targeting ctnnb2. B. Injection of wild-type ctnnb2-myc mRNA at the 1-cell stage could efficiently rescue the ventralized phenotypes. C. Schematics shows the mutation types by examining colonies.
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