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Fig. 5

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ZDB-IMAGE-241203-34
Source
Figures for Zhang et al., 2024
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Figure Caption

Fig. 5 Golden Gate cloning of tandem sgRNA expression vector and Gibson assembly of double-genes sgRNA expression cassettes. A. BsaI digestion leaves specific overhangs, which are designed for tandem ligation into pGGDestISceIEG-4sgRNA. B. The amplified sgRNA expression cassette targeting gene b can be cloned into the vector of gene a through Gibson assembly.

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