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Fig. 2 Example output from CRISPRScan. Zebrafish-Danio rerio, Cas9-NGG, Gene, in vitro T7 promoter, and 4 mismatches should be chosen, and the gene symbol is then submitted to ?Get sgRNAs?. This protocol uses Cas9 for genome editing, so we select Cas9-NGG to specify the prediction of sgRNA for Cas9. Allowing for ?4 mismatches? enables the sgRNA site to tolerate up to four mismatches in off-target sequences. This criterion helps to identify a broader range of potential target sites for consideration.
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