Expression of shhaegfp and shhbegfp in zebrafish, as well as shhaegfp in medaka, during paired fin development. (A-H) EGFP expression in pectoral fin development of shhaegfp (A-D) and shhbegfp (E-H) zebrafish. (I-N) EGFP expression in pelvic fin development of shhaegfp (I-K) and shhbegfp (L-N) zebrafish. (O-R) EGFP expression in pectoral fin development of shhaegfp medaka. (S-V) EGFP expression in pelvic fin development of shhaegfp medaka. All observations were conducted on ten or more larvae at each developmental stage. Magenta fluorescence indicates paired fin skeleton marked by sox10:DsRed. Filled white arrowheads denote EGFP expression and unfilled white arrowheads denote no EGFP expression. Dashed lines outline paired fin buds. Asterisks highlight EGFP expression at the tips of fin rays. White arrows indicate autofluorescence by leucophores. Double arrows mark the anterior (A)-posterior (P) axis. The standard length of individuals (in mm) is shown in the bottom left of each panel. wpf, weeks post-fertilization. Scale bars: 100 µm (A-H); 250 µm (I-V).
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