Figure 2

Figure 2

The rate of delayed hair cell loss is dependent on initial gentamicin concentration. Fish were treated with doses of gentamicin (25, 50, 100, and 200 μM) for 1 h (red bar), then rinsed and incubated in fresh medium (blue bar). Loss of hair cells was assessed at 5, 11, 17, and 23 h after the 1 h incubation period. Increasing initial dose results in more rapid delayed hair cell loss. Differences between 25, 50, and 200 μM treatments were significant (Tukey's multiple comparison, p < 0.0001). There was no significant difference between 100 and 200 μm treatments. n = 9–11 fish, 4 NMs/fish for each treatment. Error bars represent Standard Deviation.