Fig. 7.

Fig. 7.

Abnormal swimming behaviour in JBTS mutants. (A) Plot showing the mean distance travelled during three sequential 5-min periods of darkness, light, darkness in mz cc2d2a^−/− and milder zygotic cc2d2a^−/− larvae compared to controls. The distance moved by each larva is recorded during 10 s intervals, and this distance is plotted over time. Note that both mz cc2d2a^−/− and zygotic cc2d2a^−/− larvae travel less distance than controls during both dark periods. Error shown with the coloured areas represent the 95% CI. (B) Scatter plot showing the total distance travelled during each entire 5-min period of darkness, light and darkness in mz cc2d2a^−/− and zygotic cc2d2a^−/− larvae compared to controls. The total distance travelled is significantly reduced in both mz cc2d2a^−/− and zygotic cc2d2a^−/−. Error bars are mean±s.d. ns, not significant; *P≤0.05; ****P≤0.0001. Kruskal–Wallis test with post-hoc Dunn's multiple comparisons test. Control n=46 (N=3), mz cc2d2a^−/− n=16 (N=1), zygotic cc2d2a^−/− n=32 (N=2) larvae. (C) Similar plot as in (A) but for zygotic talpid3^−/− larvae compared to controls. The distance travelled throughout the experiment does not differ between mutants and controls. Error shown with the coloured areas represent the 95% CI. (D) Similar plot as in (B) but for zygotic talpid3^−/− larvae compared to controls. The total distance is not significantly reduced in talpid3^−/− larvae (trend towards decrease in the second dark phase not significant). Error bars are mean±s.d. ns, not significant. Unpaired t-test. Control n=51 (N=3), talpid3^−/− n=37 (N=3) larvae. (E) Representative 1-min traces showing locomotion in one control, one mz cc2d2a^−/−, one zygotic cc2d2a^−/− and one talpid3^−/− larva. Black traces represent inactivity, green traces represent normal activity and red traces represent fast activity. (F) Representative montages of the touch-evoked escape response (TER) in control, zygotic cc2d2a^−/− and talpid3^−/− larvae at 2 dpf. Insets show magnified view of the larvae. While the control and talpid3^−/− larvae are able to escape the stimulus, the abnormal swimming of the zygotic cc2d2a^−/− larvae (circular with partial swimming on the side or upside down) prevents it from escaping from the stimulus in the same manner. Please note the only mild curvature of the larva, which should not suffice to explain the tightly curved swimming trace observed. (G) Scatter plot showing displacement of control, zygotic cc2d2a^−/− and talpid3^−/− larvae during the TER. Zygotic cc2d2a^−/− exhibit a significantly reduced displacement compared to controls (P<0.0001). The trend towards decreased displacement in talpid3^−/− does not reach statistical significance (P=0.1146). Error bars are mean±s.d. not significant; **** P≤0.0001. Ordinary one-way ANOVA with post-hoc Dunnett's multiple comparisons test. Control n=32 (N=4), zygotic cc2d2a^−/− n=20 (N=2), talpid3^−/− n=14 (N=2) larvae. N denotes the number of independent experiments (i.e. larvae from independent clutches) used for analysis.