Histological observations of male zebrafish testes after 2-month in vivo exposure to DBDPE. (A−E) Typical hematoxylin/eosin–stained sections of testes in 0, 0.1, 1, 10, and 100 nM-exposure groups, respectively. (F) Proportion quantification of germ cells at different developmental stages in testes (n=6–9 fish/group). Results are represented as means±standard errors of the mean (SEMs). Scale bar: 50μm. Data are reported in Excel Table S3. Note: DBDPE, decabromodiphenyl ethane; SPC, spermatocytes; SPD, spermatozoa; SPG, spermatogonia. *p<0.05, **p<0.01, and ***p<0.001 indicate significant differences between exposure and control groups, by one-way analysis of variance (ANOVA) followed by the post hoc least significant difference (LSD) test.
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