Fig 7

Fig 7 Mitoxantrone inhibits αMSH-induced physiological pigmentation.

(A) Representative traces of Fura-2 imaging to measure cytosolic Ca²⁺ in control and mitoxantrone pretreated B16 cells stimulated with 1 μm αMSH. (B) Quantitation of cytosolic Ca²⁺ levels in control and mitoxantrone pretreated B16 cells stimulated with 1 μm αMSH where “n” denotes the number of ROIs. (C) Representative mitochondrial Ca²⁺ imaging traces of CEPIA2mt in control and mitoxantrone pretreated B16 cells stimulated with 1 μm αMSH. (D) Quantitation of mitochondrial Ca²⁺ uptake by calculating increase in CEPIA2mt signal (ΔCEPIA2mt) in control and mitoxantrone pretreated B16 cells stimulated with 100 μm histamine where “n” denotes the number of ROIs. (E) Representative pellet pictures upon αMSH, αMSH+mitoxantrone treatment in B16 cells as compared to vehicle control. (F) Mean pixel intensity of αMSH, αMSH+mitoxantrone treatment in B16 cells as compared to vehicle control (N = 3). Data presented are mean ± SEM. For statistical analysis, unpaired Student’s t test was performed for panels B and D, while one-way ANOVA followed by Tukey’s post hoc test was performed for panel F using GraphPad Prism software. Here, * p < 0.05; ** p < 0.01; *** p < 0.001; and **** p < 0.0001. The data underlying for panels A, B, C, D, and F shown in the figure can be found in S1 Data.