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Fig. 2 H2O2 induces nociceptive-like behaviour in zebrafish larvae via TRPA1. (a) Typical traces, concentration?response curve and cumulative data of calcium responses in zTRPA1b-HEK293T cells exposed to H2O2 or vehicle (Veh) and in the presence of A-967079 (A96, 30 ?M) (cell numbers: H2O2: 3 ?M n = 120, 10 ?M n = 93, 30 ?M n = 78, 100 ?M n = 81, 300 ?M n = 90, 1 mM n = 83, 3 mM n = 90, 10 mM n = 99, A96 = 110, Veh = 77). (b,c) Typical traces and cumulative data of the locomotor activity of 5 days post fertilization (dpf) zebrafish larvae exposed to H2O2 (100?500 ?M, 1 mM) or Veh and in the presence of A96 (10 ?M) (n = 8?15 larvae per group). (d) Typical images and dose-dependent writhing behaviour in 24?27 post fertilization (hpf) zebrafish larvae exposed to H2O2 (30?100?300 mM) or Veh and in the presence of A96 (30 ?M) (n = 60 larvae per group). (e) Typical images and dose-dependent light touch response in 24?27 hpf zebrafish larvae exposed to H2O2 (30?100?300 mM) or Veh and in the presence of A96 (30 ?M) (n = 60 larvae per group). Mean ± SEM. *P < 0.05; #P < 0.05 one-way ANOVA and Bonferroni post hoc test.