Fig. 8 CNCCs migration in PB (200 nM)-treated sox10:EGFP embryos at 13 ss. (a?b) Overall defects were analyzed at 13 ss. (c?d?) Live DIC (differential interference contrast, (c), (d)) and fluorescence imaging (c?, d?) of CNCC migration in control and PB-treated embryos. The yellow arrowheads in (c) and (d) indicate the position of the midbrain-hindbrain boundary (MHB) at which CNCCs start migration. The white arrowhead in (c?) and (d?) indicates the frontier of migratory CNCCs via the frontonasal pathway. Dashed line indicates the eye (e). Scale bars: 100 ?m. n = 30 for each experiment. All experiments were performed in three biological replicates.
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