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Fig. 5 Morphological phenotype resulting from PB treatment in zebrafish embryos at 24 hpf. (a?d) Comparison of overall morphology and body length between control embryos and PB-treated embryos. Black line in the panel (a) indicates the measured length from the head to tail. (e) Quantification of the length from the head to tail. ***p < 0.001 (one-way ANOVA followed by Dunnett's multiple comparison test). (f?m?) Effects of PB (200 nM) on neural crest cells in the pharyngeal arch (PA)s which is addressed by the yellow arrowheads (g?k?) and major axonal trajectories and commissure neurons (h?i?). The trigeminal ganglion is marked by the white arrowhead. Brain (midbrain) is indicated by the bracket ((i), (m)), and eye is indicated by the arrowhead ((i?), (m?)) were analyzed. n = 30 for experiment for (f?i) and (j?m). (n) Quantification of the length of the midbrain shown in panels (i) and (m). ***: p < 0.001 (one-way ANOVA followed by Dunnett's multiple comparison test). Scale bar: 100 ?m. The dots in (e) and (n) represent individual embryos examined. All experiments were performed in three biological replicates.