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Fig. 1 AITC and 4-HNE promote nociceptive-like behaviour in zebrafish larvae via TRPA1. (a,b) Typical traces, concentration?response curve and cumulative data of calcium responses in zTRPA1b-HEK293T cells stimulated with AITC, 4-HNE or vehicle (Veh) and in the presence of A-967079 (A96, 30 ?M) (cell numbers: AITC: 1 nM n = 96, 10 nM n = 75, 100 nM n = 112, 1 ?M n = 70, 3 ?M n = 111, 10 ?M n = 94, 100 ?M n = 80, 1 mM n = 84, A96 = 85, Veh = 44; n = 3 independent experiments) (cell numbers: 4-HNE: 300 nM n = 56, 1 ?M n = 52, 3 ?M n = 90, 10 ?M n = 77, 30 ?M n = 80, 100 ?M n = 98, 300 ?M n = 92, 1 mM n = 64, A96 = 88, Veh = 77). (c?f) Typical traces and cumulative data of the locomotor activity of 5 days post fertilization (dpf) zebrafish larvae exposed to AITC (0.01?0.1?1?10 ?M), 4-HNE (1?10?100 ?M) or Veh and in the presence of A96 (10 ?M) (n = 8?16 larvae per group). (g,h) Typical images and dose-dependent writhing behaviour in 24?27 post fertilization (hpf) zebrafish larvae exposed to AITC (50?100?500 ?M), 4-HNE (100?500 ?M, 1 mM) or Veh and in the presence of A96 (30 ?M) (n = 60 larvae per group). (i,j) Typical images and dose-dependent light touch response in 24?27 hpf zebrafish larvae exposed to AITC (50?100?500 ?M), 4-HNE (100?500 ?M, 1 mM) or Veh and in the presence of A96 (30 ?M) (n = 60 larvae per group). Mean ± SEM. *P < 0.05; #P < 0.05, one-way ANOVA and Bonferroni post hoc test.