ZFIN Image: Wu et al., 2024, Figure 2

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Figure Caption

Figure 2

Pretreatment with SFN showed no protective effect against ethanol-induced teratogenicity. (A) Zebrafish embryos were treated with 2 µM SFN from 3 to 24 hpf, then exposed to 1.5% ethanol from 24 to 48 hpf, and thereafter incubated in fresh egg water. (B) Survival rate. (C) Hatching rate. (D) Body length. (E) Representative larvae of control without treatment (first row), 3–24 hpf treatment with 2 µM SFN (second row from top), 24–48 exposure with 1.5% ethanol (third row), and 3–24 hpf pretreatment with 2 µM SFN and then 24–48 exposure with 1.5% ethanol (bottom row). (F) Histogram (next to the larvae images) indicates the percentage of larvae with typical FASD malformations. Arrows indicate the phenotypes of FASD. se, small eyes; rb, reduced brain; sov, small otic vesicle; sj, small jaw; and pe, pericardial edema. One-way ANOVA was used to analyze the effects of SFN alone and t-test for pairwise comparison with ns, not significant, * p < 0.05, ** p < 0.01, and **** p < 0.0001. The scale bar represents 1 mm.

Acknowledgments

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