ZFIN Image: Ghasempour et al., 2024, Figure S2.

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Figure Caption

Figure S2.

Molecular characterization of the zebrafish itgav gene and generation of itgav knockout mutant. (A) The zebrafish itgav gene: The genomic structure of the zebrafish itgav gene, comprising 30 exons, is depicted. (B)itgav^−/− mutant generation: CRISPR/Cas9 genome editing was employed to generate an itgav^−/− mutant. The guide RNA targeted exon 4 just before the conserved tryptophan, resulting in a 3-bp deletion and an early stop codon. (C) Molecular validation: HRM analysis corroborated the deletion, and real-time PCR demonstrated downregulation of itgav mRNA, suggesting nonsense-mediated RNA decay.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Exp. Med.