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Fig. 5 HAX1 regulates vascular morphogenesis in zebrafish. (a) In situ hybridization was performed on zebrafish embryos at 12 hpf, 20 hpf, 24 hpf and 60 hpf to observe the HAX1 level. (b) HAX1 mRNA was injected in the embryo. Left, representative pictures of the sub-intestinal vessel using confocal microscopy. Right, stroke outlines of the sub-intestinal vessel. (c, d) Reduced HAX1 level. Left, representative pictures of (c) the intersegmental vessel and (d) head vessel. Right, stroke outlines of the intersegmental vessel. (e) Top: CRISPR/Cas9 targeting sequences. Down, representative pictures of the intersegmental vessel, bar: 20 µm. (***p < 0.001, Student's t-test)