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Fig. 6 Confocal analysis at 24 h post-transfection (T0). The plasmid containing rack1+ and the reporter hemagglutinin (RACK1+/HA) produced green fluorescence. The cells were double-labelled with embryonal markers (GATA4, WT1, and NFAT2; red fluorescence) and compared to nontransfected cells (CTR). GATA4 is increased in transfected cells and is localized mainly in the cytoplasm. WT1 is comparable to the CTR. NFAT2 is sparsely expressed in the CTR and, in both cases, is localized in the nucleus. The nucleus was labelled with DAPI (blue fluorescence). DAPI/Ab: DAPI-labelled antibody (GATA4, WT1 or NFAT2). TRANSF/MERGE: Transfected cells labelled with all the antibodies (against HA and one embryonal marker). CTR/MERGE: nontransfected cells labelled with all the antibodies. The hearts utilized for the experiments were N = 4/5 sections in each group in triplicate experiments. The hearts utilized for the experiments were N = 3, and analysis was performed with 104 cells in each group in triplicate. Bar: 20 μm.