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Fig. 4 Homozygous chchd10P83L larvae do not display expression or assembly defects of mitochondrial respiratory chain subunit complexes or differences in expression of transcripts involved with the ISR. Ai, Representative immunoblot of mitochondrial respiratory chain subunits in 5 dpf wild type and chchd10P83L/P83L larval lysate. Aii, No significant differences were found between wild type and chchd10P83L/P83L larvae in the expression of any of the respiratory chain subunits: Ndufa9 (t (4) = 0.89, p = 0.44); mt-Co1 (t (4) = 0.006, p = 0.95); Uqcrc2 (t (4) = 1.07, p = 0.35); Atp5a (t (4) = 1.42, p = 0.23). Sdhb was used as loading control. N = 3 per genotype. Bi, Representative Blue Native PAGE in 5 dpf wild type and chchd10P83L/P83L larvae using lysate from one larva in each lane. Bii, No significant differences were found between wild type and chchd10 P83L/P83L larvae in the assembly levels of any of the complexes. Complex I (t (10) = 1.42, p = 0.18); Complex III (t (10) = 0.455, p = 0.66); Complex IV (t (8) = 0.46, p = 0.66); Complex V (t (10) = 1.07, p = 0.31). Sample sizes are n = 6 per genotype, except Complex IV (n = 5 per genotype). C, Transcripts related to the ISR were not significantly altered in chchd10P83L/P83L larvae: atf4a (t (4) = 2.10, p = 0.10); atf4b (t (4) = 1.565, p = 0.19); atf5a (t (4) = 0.5494, p = 0.61); atf5b (t (4) = 0.8625, p = 0.44), and chop (t (4) = 0.745, p = 0.49). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)