Figure 2.

Figure 2.

BoTx-mediated inhibition of GCs suppresses orienting behavior. Diagrams for the expression pattern of BoTx in adult transgenic zebrafish gSA2AzGFF152B;Tg(UAS:BoTxBLC-GFP) (A) and Tg(cbln12:Gal4FF); Tg(UAS:BoTxBLC-GFP) (B). CC, crista cerebellaris; CCe, corpus cerebelli; CrC, crest cell; EG, eminentia granularis; GL, granular layer; LCa, lobus caudalis cerebelli; ML, molecular layer; SM, stratum marginale; TeO, tectum opticum; TL, torus longitudinalis; Va, valvula cerebelli. Orienting behavior of 152B::BoTx, C, E, F, I, and J or cbln12::BoTx, D, G, H, K, and L, which express BoTx in GCs. Sibling zebrafish that did not express BoTx were used as controls. Representative traces and polar histograms for 152B::BoTx and its control (C) and cbln12::BoTx and its control (D). C–H, Orienting behavior in two large tanks. The same type of test fish (either BoTx-expressing or control fish) were placed in both tanks. I–L, Orienting behavior toward wild-type control fish. A combination of standard and short tanks was used. The test fish was placed in the standard tank, while wild-type fish was placed in the short tank (for visual stimulus). The orienting behavior of the test fish in the one tank (E–H; large tank for I–K, H) was analyzed. Percentages of the time spent in the ROI (E, G, I, K) and percentages of the time that fish showed the orienting angles in the ROI (F, H, J, L) are indicated. 152B::BoTx spent less time in the ROI in the two large tank (n = 20 each in E, F) and large/small tank (n = 10 each in I, J) assays. cbln12::BoTx spent less time in the ROI and exhibited the orienting angles for a shorter duration than controls in the two large tank (control n = 20; cbln12::BoTx n = 24 in G, H) assays. Representative structures of time lag cross-correlation for 152B::BoTx and control fish (M) and cbln12::BoTx and control fish (O). Black bars indicate latency from time 0 to peak. Quantification of latency to peak correlation in 152B::BoTx and control fish (N) and cbln12::BoTx and control fish (P). Latency to peak correlation in cbln12::BoTx was significantly longer than that in control. See Extended Data Figure 2-1 for more details.