Figure 5.

Figure 5.

The cerebellum is activated during orienting behavior. A, Detection of neuronal activity during orienting behavior. After 20 min of acclimatization, zebrafish were allowed to swim for 5 min under no-stimulus condition, followed by 5 min of orienting behavior assays under no-stimulus or stimulus conditions. For RT-qPCR, zebrafish were further kept for 15 min under no-stimulus condition, and then RNA from the cerebellum was isolated. B, C, c-fos (B) and egr1 (C) expressions detected by RT-qPCR. c-fos and egr1 transcripts were amplified from cDNAs from the cerebellum of zebrafish under no-stimulus or stimulus conditions. Data are shown as ratios to expression in fish under no-stimulus conditions. Expressions of c-fos and egr1 mRNA significantly increased in the cerebellum of zebrafish that exhibited orienting behavior under stimulus conditions, compared with no-stimulus conditions (B, c-fos n = 5, C; egr1 n = 5). See Extended Data Figure 5-1 for more details.