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Fig. 3 Fasting increases heat resistance and induces mitochondrial fission in adult zebrafish. (A) Representative images of zebrafish liver mitochondrial morphology after the indicated treatment. The fast group was not fed for 2 days; 0.5 mM CQ was treated for two days. Scale bar = 5 µm. (B) Percentile of cells with fragmented mitochondria. **indicates p < 0.01. (C) Survival rate of the indicated three groups to heat tolerance test. n = 20 per experiment, repeat = 3. Statistical significance was determined between the fed and the fast groups, and between the fast and the fast + CQ groups as indicated. *indicates p < 0.05. CQ, chloroquine.