Figure 1

Figure 1

Lethality and sub-lethal effects of zebrafish embryos exposed to various concentrations of A. pseudoplatanus toxins, hypoglycin A, and methelynecyclopropylacetate over 72 h. The experiment was conducted to determine the median lethal (LC50) and median sub-lethal (EC50) concentration for hypoglycin A (A) and methelynecyclopropylacetate (B) at 24, 48, and 72 h post-exposure. Data were obtained from three independent replicates with 20 embryos per concentration per replicate. The LC50 and EC50 values were calculated using a four-parameter log-logistic model implemented with the drc R package, along with the associated standard error and the p-value. The sample size was 60 individuals per concentration (3 trials × 20 individuals), and the statistical test was a logistic regression with four parameters.