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Fig. 2 Heat acclimation reduces the expression of functional mitochondrial genes and induces mitochondrial fission. (A) Expression levels of genes related to mitochondrial function (atp5f1b, ndufa9a, and cpt1ab) in the liver were quantified and graphed. n ≥ 7 per experiment, repeat = 2. *indicates p < 0.05. (B) Representative images of liver mitochondrial morphology after 2 months of heat stress at 33°C. Scale bar = 5 µm. (C) Percentile of cells with fragmented mitochondria. **indicates p < 0.01.
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