Figure 6

Figure 6

siRNAs designed to target zebrafish-specific lncRNA BX323557.1 prompt apoptosis or pyroptosis in human CRC cells (A) Representative microscopic pictures of HCT116 and DLD1 cells treated with siBX323557.1. Apoptotic and pyroptotic cells were denoted by red or yellow arrowheads, respectively. Scale bar, 100μm. (B) Comparison of LDH levels in the cultural supernatant between two human CRC cell lines. (C) Morphologies observed by the transmission electron microscope of HCT116 cells under indicated treatments. Scale bar, 2μm. (D, E) The expression of protein PARP, GSDME, caspase-3, and the corresponding cleaved segments in HCT116, DLD1, and NCM460 cells treated with specified siRNAs. (F) Quantified statistics of immunoblotting analyzed by Image J. The level of α-tubulin is applied as an internal control. All the data are presented in the format of mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns, no significant difference.