Figure 1

Figure 1

siRNAs designed to target mouse-specific lncRNAs lead to cell death in human CRC cells (A, B) Representative microscopic graphs of HCT116, DLD1, and CT26 cells taken 48 hours after transfection with siNC, siRNAs targeting mouse-specific lncRNAs Gm38195 or Gm48223, respectively. Apoptotic and pyroptotic cells were denoted by red or yellow arrowheads, respectively. “siNC” was a 19bp siRNA designed by RiboBio with no targeting sequence, acting as a negative control. “siRNA-1/2/3” meant three 19bp siRNAs targeting different sequences of the lncRNA as indicated. (C, D) Percentages of the area covered by living cells under indicated treatments, three independent perspectives were randomly selected for measurement by using Image J. Scale bar, 100μm. (E -H) Total cell death was determined by flow cytometry. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns, no significant difference.