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Fig. 4 Comparison of DTI results in the main brain regions of tlr2−/− and control adult zebrafish. Representative central slice of DTI measurements of control and tlr2−/− adult zebrafish. (A) From these slices, the diffusion tensor metrics were estimated: D∥, D⊥, MD and FA, as well as (B) the diffusion kurtosis metrics: K∥, K⊥, MK and KFA. (C) DTI and DKI results of tlr2−/− adult zebrafish compared with controls reveal a noteworthy trend towards decreased D∥, D⊥ and MD, reduced FA, as well as increased K∥, K⊥ and MK. Additionally, a significant decrease in KFA is observed in the tlr2−/− group. Acquisition details: TR 2000 ms, TE 12.4 ms, 32 averages, resolution 25 × 25 × 200 μm, effective b-value range 4, 1000, 3500 or 6000 s/mm2, with 8, 12, 24 or 36 directions, respectively. Statistical analysis was performed using the unpaired t-test, assuming Gaussian distribution, with p less than 0.05 considered to imply significant differences between the control and the tlr2−/− group. *p < 0.05, ***p < 0.005. CB, cerebellum; D∥, axial diffusivity; D⊥, radial diffusivity; DC, diencephalon; DKI, diffusion kurtosis imaging; DTI, diffusion tensor imaging; FA, fractional anisotropy; K∥, axial kurtosis; K⊥, radial kurtosis; KFA, kurtosis fractional anisotropy; MC, mesencephalon; MD, mean diffusivity; MK, mean kurtosis; OB, olfactory bulb; Pa, pallium; RC, rhombencephalon.