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Fig. 3 Phasor analysis of the experimental ADC curves. (A) Phasor plot of the CCem of tlr2−/− and control, relative to the phasor plot semicircle (red). On the semicircle, 0 mm2/s is located at [0,0], with steps of 1·10−4 mm2/s indicated as red dots on the semicircle. (B) Monocomponent analysis shows that the ADC in tlr2−/− is significantly reduced, while (C–E) Multicomponent analysis of the ADC decay curve in the CCem shows a significant increase in the area of the slow diffusion component in tlr2−/−, associated with restricted diffusion. Acquisition details for the DWI used for phasor plot analysis: TR 1000 ms, TE 13 ms, 4 averages, resolution 47 × 47 μm, slice thickness 200 μm and effective b-value range 50, 500, 1000, 1500, 2000, 2500 or 3000 s/mm2. Statistical analysis was performed using the unpaired t-test, assuming Gaussian distribution, with p less than 0.05 considered to imply significant differences between the control and the tlr2−/− group. * p < 0.05. ADC, apparent diffusion coefficient; CCem, molecular layer of the cerebellar corpus; DWI, diffusion-weighted imaging.