Figure 6

Figure 6

The effect of overexpressing mutant Eno2 on the occurrence rate of branched axons of motor neurons in zebrafish embryos immersed with recombinant Pgk1. (A,B) Two phenotypes of the caudal primary (CaP) axons of motor neurons in the 30 hpf transgenic line Tg(mnx1:GFP) embryos. (A) Examples of normal phenotype and (B) branched axon phenotype (branching sites indicated by white arrowheads). Scale bar: 50 μm. Experimental manipulations were performed at the one-cell stage. The first group of zebrafish embryos served as the non-injected control group, the second group was injected with mouse eno2-wb mRNA, and the third group was injected with mutated eno2-wb mRNA. At 30 hpf, the percentage of embryos exhibiting the branched axonal phenotype among CaP neurons was calculated for each group. (C) The results of embryos injected with eno2-wb-[D419] mRNA and (D) the results of embryos injected with eno2-wb-[E420K] mRNA. Each panel represents the average of three independent experiments with statistical analysis performed using one-way ANOVA (*, p < 0.05; ns indicates no significant difference). F statistics of (C) were 1.239 as numerator and 2.478 as denominator, while (D) were 1 as numerator and 2.001 as denominator.