ZFIN Image: Thomas et al., 2024, Figure 5.

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Figure Caption

Figure 5.

Embryos from older rnaseh2a^−/− fish are significantly underdeveloped compared with embryos from younger fish. (A) Movement analysis of adult zebrafish revealed the distance moved by rnaseh2a^−/− is significantly shorter than rnaseh2a^+/+ adults. Unpaired t-test, n = 10, individual adults, ±SD, ** P < 0.001. (B) Photomoter response of embryos from rnaseh2a^+/− parents show no significant difference in distance moved by rnaseh2a^+/+ compared with rnaseh2a^−/− ±SD, unpaired t-test, n = P < 0.05. (C) Representative images of embryos from 6 month old and 12 month old rnaseh2a^−/− zebrafish. (D) Quantification of embryo length from old and young zebrafish n = 36, individual embryos. Unpaired t-test **** P < 0.0001. (E) RTqPCR showing an increase in the expression level of ISG15 in embryos resulting from older rnase2a^−/− adults. n = 3, 20 pooled embryos. Unpaired t-test P < 0.05.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.