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Fig. 5 The epoa mRNA overexpression rescued anemia in epoaszy8. (A) WISH of 4-dpf larvae injected with epoa mRNA. Larvae of epoaszy8 are completely rescued by epoa mRNA to WT level. Representative images are shown for staining of ?e1-globin (indicated by red triangular arrowheads) in 4-dpf WT and epoaszy8 larvae. (B) Relative expression of erythroid cells markers in 4-dpf WT control group (blue column), WT epoa mRNA injection group (WT + epoa, purple column), epoaszy8 control group (red column) and epoaszy8 + epoa group (orange column) quantified by RT-qPCR. Statistical significance was determined using a two-sample Student's t-test, n ? 15 per group, and data were combined from four replicates, mean ± SEM, ns: no significance, *p < .05, **p < .01, ***p < .001 and ****p < .0001. (C) Relative expression of epob in 4 dpf WT and epoaszy8 quantified by RT-qPCR. Statistical significance was determined using a two-sample Student's t-test, n ? 15 per group, mean ± SEM, ns: no significance.