|
Fig. 7 E-cadherin is responsible for endodermal C&E defects in gna13a/gna13b-deficient embryos. (A) Schematic diagram illustrating endoderm transplantation procedure. Donor embryos were injected with sox32 RNA (which confers endodermal identity to all cells), rhodamine-dextran (a lineage tracer) and a control p53 MO or MOs targeting cdh1 and p53 at the one-cell stage. At the sphere stage, about 50 donor cells were transplanted into host Tg(sox17:EGFP) embryos (endoderm is labeled with EGFP). (B-E) Snapshots from Movie 3 showing the pharyngeal EGFP-labelled endoderm in control Tg(sox17:EGFP) hosts transplanted with rhodamine-labeled donor cells (magenta) at the beginning (0 min, 7s) and end (140 min, 10s) of the movie. (B,C) Control MO-injected cells. (D,E) cdh1 MO-injected cells. Magenta and green lines indicate the widths of the donor and host endodermal sheets, respectively. Dorsoanterior view with anterior upwards. A, anterior; P, posterior; ML, mediolateral. (F) Average convergence velocity of donor and host endoderm in indicated embryos in B-E with the number of embryos indicated. Data were generated from four experiments. (G-J) Epifluorescence images of the pharyngeal endoderm in fixed Tg(sox17:EGFP) embryos at 11 ss that were uninjected or injected with cdh1 RNA. Yellow asterisks indicate endodermal holes. Yellow lines (equivalent length) indicate the width of the pharyngeal endoderm sheet. Dorsoanterior view with anterior upwards. (K) Average width of the pharyngeal endoderm in the indicated embryos shown in G-J, from three experiments. The number of embryos analyzed in each group is indicated. Data are mean±s.e.m. n.s. (not significant), P>0.05; ****P<0.0001 (unpaired, two-tailed Student's t-test).