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Fig. 3 Dnajc21 loss impairs myeloid and erythroid differentiation.Brightfield images of whole mount in situ hybridization for (A) myb+ hematopoietic stem and progenitor cells at 36 hpf; the ventral wall of the dorsal aorta is outlined and shown at higher magnification. B lcp1+ total leukocytes and mpx+ neutrophils during definitive myelopoiesis at 48 hpf; and C hbbe3+ mature erythrocytes during definitive erythropoiesis at 48 hpf. Lateral views are shown with anterior to the left. Numbers on the lower right indicate the number of embryos with the same phenotype. Experiments were done in 2–4 biological replicates, each comprising at least 20 embryos per genotype. The black dotted box marks the region in the trunk used for counting mpx+ neutrophils. Graphs show quantification of cell counts or staining pattern per embryo. hpf: hours post-fertilization. ****p < 0.00001.