PUBLICATION
Loss of Dnajc21 leads to cytopenia and altered nucleotide metabolism in zebrafish
- Authors
- Ketharnathan, S., Pokharel, S., Prykhozhij, S.V., Cordeiro-Santanach, A., Ban, K., Dogan, S., Hoang, H.D., Liebman, M.F., Leung, E., Alain, T., Alecu, I., Bennett, S.A.L., Čuperlović-Culf, M., Dror, Y., Berman, J.N.
- ID
- ZDB-PUB-240814-8
- Date
- 2024
- Source
- Leukemia 38(10): 2115-2126 (Journal)
- Registered Authors
- Ban, Kevin, Berman, Jason, Ketharnathan, Sarada, Prykhozhij, Sergey
- Keywords
- none
- Datasets
- GEO:GSE225613
- MeSH Terms
-
- Animals
- Cell Differentiation
- Cell Proliferation
- Cytopenia
- DNA Damage
- Hematopoiesis
- Mutation
- Nucleotides/metabolism
- Tumor Suppressor Protein p53/genetics
- Tumor Suppressor Protein p53/metabolism
- Zebrafish*
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- PubMed
- 39138265 Full text @ Leukemia
Citation
Ketharnathan, S., Pokharel, S., Prykhozhij, S.V., Cordeiro-Santanach, A., Ban, K., Dogan, S., Hoang, H.D., Liebman, M.F., Leung, E., Alain, T., Alecu, I., Bennett, S.A.L., Čuperlović-Culf, M., Dror, Y., Berman, J.N. (2024) Loss of Dnajc21 leads to cytopenia and altered nucleotide metabolism in zebrafish. Leukemia. 38(10):2115-2126.
Abstract
Mutations in the DNAJC21 gene were recently described in Shwachman-Diamond syndrome (SDS), a bone marrow failure syndrome with high predisposition for myeloid malignancies. To study the underlying biology in hematopoiesis regulation and disease, we generated the first in vivo model of Dnajc21 deficiency using the zebrafish. Zebrafish dnajc21 mutants phenocopy key SDS patient phenotypes such as cytopenia, reduced growth, and defective protein synthesis. We show that cytopenia results from impaired hematopoietic differentiation, accumulation of DNA damage, and reduced cell proliferation. The introduction of a biallelic tp53 mutation in the dnajc21 mutants leads to the development of myelodysplastic neoplasia-like features defined by abnormal erythroid morphology and expansion of hematopoietic progenitors. Using transcriptomic and metabolomic analyses, we uncover a novel role for Dnajc21 in nucleotide metabolism. Exogenous nucleoside supplementation restores neutrophil counts, revealing an association between nucleotide imbalance and neutrophil differentiation, suggesting a novel mechanism in dnajc21-mutant SDS biology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping