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Fig. 2 dnajc21 mutants show reduced global translation.A Representative polysome profiles of wildtype and dnajc21−/− mutant embryos at 48 hpf. B Immunoblotting following puromycin incorporation into nascent proteins in wildtype and dnajc21−/− mutant embryos at 48 hpf. GAPDH was used as loading control. Intensity of puromycin signal relative to GAPDH was measured. hpf: hours post-fertilization. *p < 0.01.
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