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Fig. 6 Analysis of DEGs, GO BPs enrichment, and KEGG pathways in SHK-treated Kasumi-1 cells. (A) Kasumi-1 cells were treated with vehicle or SHK (0.2 and 0.4 ?M) for 48 h, and the mRNA expression profiles were determined using the human cDNA microarray. The Venn diagram illustrates the co-upregulated and co-downregulated DEGs in response to SHK treatment compared to the vehicle group. (B) Significantly enriched GO BP terms in cells treated with SHK. (C) KEGG pathways enriched by DEGs in SHK-treated cells. (D) Kasumi-1 cells were treated with vehicle or SHK (0.2 and 0.4 ?M) for 48 h. The protein levels of p53 and ?-actin were determined by Western blot analysis. A representative blot is shown. (E) Normalized intensity of p53 versus ?-actin proteins. Data represent the mean ± SD of three independent experiments. **p < 0.01 indicates significant differences compared to the vehicle-treated cells.