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Fig. 4 Effects of SHK on toxicity in zebrafish larvae. (A) Schematic illustration depicting the experimental protocol. Zebrafish larvae at 72 hpf were treated with either vehicle (DMSO) or SHK (0.2 and 0.4 ?M) in E3 medium and then incubated at 34°C for 24?72 h. Zebrafish larvae were observed after 24, 48, and 72 h of SHK treatment. (B) Morphology was observed using a microscope. Representative images are shown, and the scale bar is 500 ?m. (C) Survival percentage, (D) body length, and (E) heartbeat rate was measured as described in the Materials and Methods section. Data are presented as mean ± SD. **p < 0.01 indicates significant differences compared to the 24 hpt group, while "ns" denotes no significant difference.