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Fig. 3 Effects of SHK on AML1-ETO and c-KIT gene expression in Kasumi-1 cells. Kasumi-1 cells were treated with vehicle or SHK (0.2 and 0.4 ?M) for 48 h. The mRNA levels of (A) AML1-ETO and (D) c-KIT were determined by RT-qPCR analysis. The protein levels of (B) AML1-ETO and (E) c-KIT were detected by Western blot analysis, and a representative blot is shown. Normalized intensity of (C) AML1-ETO and (F) c-KIT versus ?-actin proteins. Data represent the mean ± SD of three independent experiments. *p < 0.05 and **p < 0.01 represent significant differences compared to the vehicle-treated cells.
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