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Fig. 2 Autophagy inhibition by atg5 depletion reveals tau toxicity in the Tau tg line. a Western blots of Ctrl and atg5 morphants at 2 dpf. atg5 morphants exhibited accumulation of LC3-I and p62 as well as total Tau and pTau (T46 and AT8). b, c Densitometry measurement of triple biologic replicates of western blots using T46 (b) or anti-LC3 (c) antibodies in (a). d Representative confocal images of primary motor axons of Ctrl and atg5 morphants of the Ctrl and Tau tg lines at 2 dpf by Znp1 immunostaining (left panels) and tracing of motor axons for quantitation using images J (right panels). e Both lengths and branches numbers of motor axons were significantly reduced in atg5 morphants of the Tau tg line, but not in Ctrl. Data represent mean ± SEM, n = 10 (10 individual animals), ANOVA test, **p < 0.01; ns not significant, Scale bar = 200 µm