Figure 2

Figure 2

TnP sustains Cyp1a expression induced by FICZ. (A) Fluorescence intensity analysis measured by ImageJ of Tg(cyp1a:EGFP) zebrafish reporter embryos treated with FICZ and/or TnP to assess cyp1a expression over time, with exposure to the compounds starting at 24 h post-fertilization (hpf). DMSO 0.01% v/v was used as a negative control. Graph produced using GraphPad Prism Software (San Diego, CA, USA). * represents a significant difference (p < 0.05) to the negative control (DMSO), and # represents a significant difference (p < 0.05) to FICZ. (B) Selected representations of Tg(cyp1a:EGFP) zebrafish reporter embryos to assess the intensity and spatial distribution of AHR activation through Cyp1a expression at 96 h post-treatment (hpt). Abbreviations: FICZ—6-Formylindolo[3,2-b]carbazole; TnP—Thalassophryne nattereri Peptide; DMSO—Dimethyl Sulfoxide.