Figure 5

Figure 5

Tg(Hsa.CTGF:nlsmCherry)^ia49 reporter activity is increased in apc mutant background at 72 hpf. (A) apc^hu745 mutants fail to develop the cartilage structure, as evidenced by the alcian blue staining. (B) The reporter signal, labeling the pharyngeal arches (ph), disappears in apc^hu745 mutant background due to the lack of these structures, as shown by the in situ hybridization against mCherry mRNA as well. (C) In situ hybridization for mCherry displaying the general upregulation of Yap/Taz reporter activity in apc^hu745 mutant background. The increase in mCherry mRNA levels is evident in the brain (b), eyes (e), otic vesicle (ov), heart (h), and vascular system (vs) along the yolk extension. (D,E) Quantification of the in-situ hybridization experiments was performed (D) by grouping the embryos into three distinct classes according to the observed signal strength at the whole-embryo level, or (E) by measuring the pixel intensity in the head region of each sample using Volocity 6.0 software. Control (n = 48), APC^−/− (n = 39). In (E), data are presented as mean +/− SEM (Mann−Whitney test). Scale bar: 500 μm; *** p < 0.001.