Fig. 4

Fig. 4

A. Diagram of omc5 gene with exons indicated as blue boxes. Locations of nonsense mutation and the sequence encoding the epitope are indicated with arrows. B. Omcin antibody staining in omc5^−/− embryos. C. Survival of omc5^−/− embryos in germ-free solution (GF) and non germ-free solution (non-GF). D. Survival of wild type embryos in germ-free solution (GF) and non germ-free solution (non-GF). E. Survival of wild type and omc5^−/− embryos in E.coli (+) solution. F. myd88 transcript measured by ddPCR in wild type (control) and omc5^−/− embryos after incubation in control solution (PAM(−)), Peptidoglycan (PG), Lipopolysaccharide (LPS) and Flagellin (Fla). G. 10 adult fish were housed in still water for 2 days. Wild type fish remained unaffected, while all omc5^−/− fish died.