Fig 5

Fig 5 Ectopic MYCN expression results in an aberrant SAP population that co-expresses NCC and neuronal markers at 4 dpf.

A) Cartoon depicting the z-slice through the SCG for quantifying individual triple positive (EGFP⁺/crestin⁺/elavl3⁺) cells. B) Percentage of triple positive cells (EGFP⁺/crestin⁺/elavl3⁺) detected in dbh:EGFP or dbh:EGFP-MYCN larvae at 4 dpf. For EGFP n = 7, EGFP-MYCN n = 10. C,D) Representative images from sections through the developing SCG in dbh:EGFP (C) or dbh:EGFP-MYCN (D) larvae at 4 dpf. WICHCR performed using HCR probes against crestin, elavl3, and with antibody against EGFP. Triple positive (EGFP⁺/crestin⁺/elavl3⁺) cells can be seen in MYCN overexpressing larvae. Markers: EGFP (green), crestin (magenta), and elavl3 (cyan). Scale bar = 10 μm. * P<0.05.